Molecular diagnostics in Pathology uses molecular techniques to determine specific abnormalities in tumor cells. Pathologists and clinical molecular biologists of Pathology-DNA cooperate intensively to determine the molecular characteristics of cells and tissues. Cells or tissue samples taken from the patient are investigated by pathologists. Subsequently, state-of-the-art laboratory techniques are used on the genetic material (DNA or RNA) by molecular biologists. This cooperation aims to find the correct diagnosis and prognosis leading to targeted therapy (personalized medicine).
Pathology-DNA possesses a lot of expertise on the presence or absence of molecular abnormalities in tumor cells. This expertise consists not only of DNA mutations in which only one or a few base pairs are altered, larger DNA fragments have been deleted or have been amplified, but it also consists of chromosomal changes or translocations. A large body of knowledge is present about how these changes in gene structure and activity are crucial for the behavior of a tumor and how these changes can be used to come to the correct diagnosis and treatment proposal. Direct contact between the clinician, pathologist and molecular biologist is pivotal.
Pathology-DNA not only invests in diagnostics but also in innovation and improvement of molecular techniques. We are intimately involved in applied scientific research in cooperation with many departments and hospitals.
Survey of laboratory techniques
A survey of the molecular techniques present at Pathology-DNA:
-In situ hybridization
-(Quantitative real-time) PCR
-High resolution melting curve analysis
-B and T-cell clonality analysis
Examples of most frequently used molecular tests at Pathology-DNA
-Detection of HPV in cervical cancer
-Detection of EGFR mutations in lung cancer
-Detection of RAS mutations in colon cancer
-Detection of ALK translocation in lung cancer
-Detection of BRAF mutation in melanoma
-Detection of Her2/neu overexpression/amplification in breast cancer
-Detection of B or T-cell clonality in non-Hodgkin lymphoma
-Detection of Microsatellite instability in colon cancer
-Check for correlation tissue and patient/misplacement studies