Molecular diagnostics in Pathology
Overview of Laboratory Techniques
An overview of the molecular techniques that are available:
- In situ hybridisation
- (quantitative real-time) PCR
- Fragment analysis
- High-resolution melting curve analysis
- Microsatellite analysis
- B-cell and T-cell clonality assay
- Sanger sequencing
- Next generation sequencing (NGS)
Molecular tests
Examples of the most frequently conducted molecular tests within Pathology-DNA
- Detection of HPV in cervical cancer
- Presence of EGFR mutation in lung cancer
- Absence of RAS mutation in colorectal cancer
- Presence of ALK translocation in lung cancer
- Presence of BRAF mutation in melanoma
- Presence of Her2/Neu overexpression or amplification in breast cancer
- Presence of B-cell and/or T-cell clonality in lymphomas
- Microsatellite instability in colorectal cancer
- Tissue mislabelling diagnostics